Journal: Oncology Reports

Article Title: Combination treatment with VPA and MSCs-TRAIL could increase anti-tumor effects against intracranial glioma

doi: 10.3892/or.2021.7937

Figure Lengend Snippet: Increased migration ability of MSCs-TRAIL toward VPA-treated tumors and VPA-induced increase of CXCR4 expression in MSCs-TRAIL. (A) Upregulation of CXCR4 expression was identified via western blot analysis following addition of VPA to MSCs and MSCs-TRAIL culture medium. β-actin was used as a loading control. (B) Effect of treatment with SDF-1 and AMD3100 on the migration of MSCs-TRAIL was examined using a Transwell migration assay. Magnification, ×100. Data are presented as the mean ± SD. *P<0.05 and **P<0.01 vs. the treatment with SDF-1 or VPA alone (one-way ANOVA with Bonferroni multiple comparison test). (C) Migratory ability of MSCs-TRAIL in response to conditioned medium from untreated or VPA-treated tumors was determined using a Transwell plate (8-µm pores). Representative photomicrographs of stained filters show migrated cells. Magnification, ×100. Data are presented as the mean ± SD. **P<0.01 vs. treatment of MSCs-TRAIL or VPA (one-way ANOVA with Bonferroni multiple comparison test). (D) To measure the in vivo migration capacity of MSCs-TRAIL, PKH67(green)-labeled MSCs-TRAIL were injected together with PKH26(red)-labeled VPA-treated MSCs-TRAIL into the contralateral hemisphere of tumors in a glioma model. Nuclei were stained with DAPI (blue). Box a and b, Tumor area; Box N, Normal area; T, tumor area. Visualization was conducted using a confocal microscope (magnification, ×100). Dotted line, tumor edge. VPA, valproic acid; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; DR, death receptor; MSCs-TRAIL, tumor necrosis factor-related apoptosis-inducing ligand-secreting human bone marrow-derived mesenchymal stem cells; SDF-1, stromal cell-derived factor-1; CXCR4, C-X-C chemokine receptor type 4; CM, conditioned medium; SFM, serum free medium.

Article Snippet: The migratory abilities of MSCs and MSCs-TRAIL were determined using Transwell plates (Costar; Corning, Inc.) that were 6.5-mm in diameter with 8-μm pore filters.

Techniques: Migration, Expressing, Western Blot, Control, Transwell Migration Assay, Comparison, Staining, In Vivo, Labeling, Injection, Microscopy, Derivative Assay

Journal: Oncotarget

Article Title: Tumor-suppressive function of UNC5D in papillary thyroid cancer

doi: 10.18632/oncotarget.21759

Figure Lengend Snippet: Transwell assay was performed to determine the migration ability of K1 (A) and TPC-1 cells (B) . Representative images showing cell migration in Transwell assay. The number of migration cells was counted in 6 randomly chosen fields and averaged for each of the triplicate wells. Data are expressed as the means±SEM of three independent experiments. “ * ” indicates P < 0.05, “ *** ” indicates P < 0.01. Representative images showing the migration of K1 (C) and TPC-1 cells (D) in the scratch wound-healing assay at 2 points.

Article Snippet: For the migration assay, infected cells were seeded into the upper chamber of a Transwell with a fibronectin-coated filter (8-mm pore size, Corning Life Sciences).

Techniques: Transwell Assay, Migration, Wound Healing Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Nerve growth factor promote osteogenic differentiation of dental pulp stem cells through MEK / ERK signalling pathways

doi: 10.1111/jcmm.18143

Figure Lengend Snippet: Nerve growth factor promoted proliferation of DPSCs. (A) The co‐culture pattern of DPSCs, HUVECs and SCs in transwell system at indicated ratio. NGF was up‐regulated in the co‐culture of DPSCs+HUVECs+SCs confirmed by western blot analysis, n = 3 (B) and qRT‐PCR analysis (C). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The six‐well plate transwell permeable supports (Corning, Tewksbury, MA, USA) with a 0.4 μm polycarbonate membrane were used in the co‐culture system to separate DPSCs, HUVECs and SCs into the different compartments.

Techniques: Co-Culture Assay, Western Blot, Quantitative RT-PCR